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Inferring gene regulatory logic from high-throughput measurements of thousands of systematically designed promoters


Eilon Sharon1,2,5, Yael Kalma1,2,5, Ayala Sharp2, Tali Raveh-Sadka1, Michal Levo1, Danny Zeevi1,2, Leeat Keren1,2, Zohar Yakhini3,4, Adina Weinberger1,2 & Eran Segal1,2


Despite extensive research, our understanding of the rules according to which cis-regulatory sequences are converted into gene expression is limited. We devised a method for obtaining parallel, highly accurate gene expression measurements from thousands of designed promoters and applied it to measure the effect of systematic changes in the location, number, orientation, affinity and organization of transcription-factor binding sites and nucleosome-disfavoring sequences. Our analyses reveal a clear relationship between expression and binding-site multiplicity, as well as dependencies of expression on the distance between transcription-factor binding sites and gene starts which are transcription-factor specific, including a striking ~10-bp periodic relationship between gene expression and binding-site location. We show how this approach can measure transcription-factor sequence specificities and the sensitivity of transcription-factor sites to the surrounding sequence context, and compare the activity of 75 yeast transcription factors. Our method can be used to study both cis and trans effects of genotype on transcriptional, post-transcriptional and translational control.


Correspondence should be addressed to E. Segal (eran.segal@weizmann.ac.il) or A.W. (adina.weinberger@weizmann.ac.il)


1 Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel.
2 Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.
3 Computer Science department, Technion, Haifa, Israel..
4 Agilent Laboratories, Santa Clara, California, USA.
5 These authors contributed equally to this work.